BioHawk 8-Channel
Collection/Bioidentification SystemBioHawk 8-Channel Collector/Bioidentifier

BioHawk® is a portable 8-channel bioassay system integrated with an aerosol collector. It is suitable for the high-sensitivity monitoring of biological agents, toxins, explosives, and chemical contaminants. Assay results are typically available in 10 to 20 minutes. BioHawk can be programmed to monitor surrounding air for aerosol threats with the built-in air sampler, and to periodically transfer a wet concentrate from the air sampler to the bioidentifier portion.

Bioassays are performed within a small disposable credit card-sized plastic assay coupon which can be used for up to10 assay procedures before being discarded. Since a single assay coupon can handle up to eight different analytes simultaneously, up to 80 individual assays can be performed before discarding or removing the coupon. Assay results are transmitted a through the touch panel LCD display, an audible alarm, a pulsating light, or by Bluetooth wireless or RS-232 link to personnel at a remote location. System operation may also be remotely controlled in real time.

Functions such as air sampling and bioidentification are performed using multi-step recipesdeveloped by Research International and stored in the system's computer memory. Users need only the most fundamental level of training since the internal processes and steps are presetthrough the built-in computerized recipes. For more advanced users, Windows-based software allows the user to develop their own customized sample collection and detection protocols.

Download BioHawk 8-Channel Collector/Bioidentifier Data Sheet

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  • Features
  • Applications
  • Resources
  • Operation
  • SW Interface
  • Specifications
  • FAQ
  • Order Parts
  • Man portable. Measures:35.6 cm W x 36.5 cm H x 17.1 cm D. Weighs less than 30 pounds.
  • Air sampler uses multi-stage, wetted-wall cyclone principle for enhanced particulate collection.
  • Air collection at 325 LPM, nominal.
  • Uses disposable wet assay coupon. Reusable up to 10 times. Eight simultaneous assays.
  • Fast assays: 10 - 15 minutes typical.
  • Auto-flush protocols for decontamination.
  • Analyte range: toxins, bacteria, spores, fungi, multi-cellular pathogens.
  • Sensitivity: analyte dependent, 1 to 10 ppb typical for toxins, 100 to 100,000 CFU/ml for bacteria.
  • Operator interface: Day / night touch screen LCD.
  • Designed to MILSPEC 810F.
  • Flash memory retains raw / processed data for over 6,000 assays
  • Medical
  • Agriculture
  • Military
  • Homeland security
  • Environmental
  • Indoor air quality

Sandwich Assays and Agents Detected with BioHawk Bioassay System

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Target Agent

Liquid Media

Approx. Detection Limit

References

Cocaine Urine 50ng/ml 6
TNT Water 440 ng/ml 5, 9, 11
RDX Water 1,000 ng/ml 5
Ovalbumin Water 5 ng/ml 18
Ricin Water <0.5 ng/ml 10, 15, 18, 19
Staphylococcal enterotoxin B Water 0.1-0.5 ng/ml 12, 15, 16, 18, 20
Cholera toxin Water 0.1-1 ng/ml 16, 20
D-dimer Blood plasma 200 ng/ml 8
Protein C Blood plasma 160 ng/ml 7
Bacillus globigii Water 2.5 x 104 CFU/ml 18, 20
Bacillus anthracis Water 30 CFU/ml Footnote (a)
Sterne strain, vegetative cells Whole blood 100 CFU/ml 18
Ames strain, irradiated spores Water 104 -105 CFU/ml 16, 18
Botulinium toxin Water 1 – 10 ng/ml 6, Footnote (a)
Erwinia herbicola Water 107 CFU/ml Footnote (a)
Yersinia pestis F1 antigen Water 1-5 ng/ml 15, 13, 18
Brucella abortus Water 7 x 104 CFU/ml 18
Francisella tularensis Water 5 x 104 CFU/ml 15, 18, 20
Escherichia coli O157:H7 Hamburger slurry 100-1000 CFU/g (direct) 2, 3, 4
  " " 0.08-0.4 CFU/g (6 hour enrichment) 17
  Raw sewage 1000 CFU/ml 16
Salmonella typhimurium Water 20,000 CFU/ml 14, 15, 16
Giardia lamblia Drinking Water 5 x 104/ml</2> 18
MS2 Water 109 pfu/ml 15
Vaccinia Water 105 pfu/ml 1
RSV Water Equiv. to std. ELISA</2p> Footnote (b)</2p>

Footnotes
(a) Private communication - G.P. Anderson, Naval Research Laboratory.
(b) Unpublished data - David McCrae & Ann Wilson, Research International.

 

REFERENCES

  1. K. A. Donaldson, M. F. Kramer and D. V. Lim, "A rapid detection method for Vaccinia virus, the surrogate for smallpox virus," Biosensors and Bioelectronics, 20, 322-327 (2004).

  2. D. R. DeMarco, and D. V. Lim, "Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguides," J. Food Prot, 596-602 (2002).

  3. D. Lim, "Rapid Biosensor Detection of Foodborne Microbial Pathogens," Microbiological methods Forum News, 18, 13-17 (June 2001).

  4. D. V. Lim, "Rapid Pathogen Detection in the New Millennium," National Food Processors Association (NFPA) Journal, 13–17 (October 2000).

  5. B. Bakaltcheva, F. S. Ligler, C. H. Patterson, and L. C. Shriver-Lake, "Multi-Analyte Explosive Detection using a Fiber Optic Sensor," Analytica Chima Acta, 399, 13–20 (1999).

  6. N. Nath and M. Eldefrawi, J. Wright, D. Darwin and M. Huestis, "A Rapid Reusable Fiber Optic Biosensor for Detecting Cocaine Metabolites in Urine," Journal of Analytical Toxicology, 23, 460–467 (1999).

  7. J. O. Spiker, K. A. Kang, W. N. Drohan, and D. F. Bruley, "Preliminary Study of Biosensor Optimization for the Detection of Protein C," Oxygen Transport to Tissue XX, Plenum Press, New York, 681-688 (1998).

  8. B. A. Rowe, et al., "Rapid Detection of D-dimer Using a Fiber Optic Biosensor," Thromb. Haemost., 79, 94–98 (1998).

  9. B. L. Donner, et al., "Transition from Laboratory to On-Site Environmental Monitoring of 2,4,6-Trinitrotoluene Using a Portable Fiber Optic Biosensor," ACS Symposium Series, 657 (Immunochemical Technology for Environmental Applications), 198–209 (1997).

  10. U. Narang, et al., "Fiber Optic-Based Biosensor for Ricin," Biosensor & Bioelectronics, 12, 937–945 (1997).

  11. L. C. Shriver-Lake, B. L. Donner, and F. S. Ligler, "On-Site Detection of TNT with a Portable Fiber Optic Biosensor," Environmental Science & Technology, 31, 837–841 (1997).

  12. L. A. Tempelman, et al., "Quantitating Staphylcoccal Enterotoxin B in Diverse Media Using a Portable Fiber Optic Biosensor," Analytical Biochemistry, 233, 50–57 (1996).

  13. K. Cao, G. P. Anderson, F. S. Ligler J. and Ezzel, "Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor," J. Clin. Microbiol. 33, 336-341 (1995).

  14. N. Nath and M. Eldefrawi, J. Wright, D. Darwin and M. Huestis, "A Rapid Reusable Fiber Optic Biosensor for Detecting Cocaine Metabolites in Urine," Journal of Analytical Toxicology, 23, 460–467 (1999).

  15. D. R. DeMarco, et al., Rapid Detection of Escherichia coli O157:H7 in Ground Beef Using a Fiber Optic Biosensor," Journal of Food Protection, 62, 711–716 (1999).

  16. D. V. Lim, "Detection of microorganisms and toxins with evanescent wave fiber-optic biosensors," Proc. IEEE 91, 902-907 (2003).

  17. T. B. Tims and D. V. Lim, "Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection," Journal of Microbiological Methods, 55, 141-147 (2003).

  18. G. P. Anderson, C. A. Rowe-Taitt, and F. S. Ligler, "RAPTOR: A Portable, Automated Biosensor," First Conference on Point Detection for Chemical and Biological Defense (October 2000).

  19. Ellen R. Goldman, Mehran P. Pazirandeh, J. Matthew Mauro, Keeley D. King, Julie C. Frey and George P. Anderson, " Phage-displayed peptides as biosensor reagents," Journal of Molecular Recognition, 13 (6), 382 – 387, 2000.

  20. G. P. Anderson, K. D. King, K. L. Gaffney, and L. H. Johnson, "Multi-Analyte Interrogation Using the Fiber Optic Biosensor," Biosensors & Bioelectronics, 14, 771–777 (2000).

  21. R. A. Ogert, et al., "Detection of Clostridium botulinium Toxin A Using a Fiber Optic-Based Biosensor," Analytical Biochemistry, 205, 306–312 (1992).

Air Sampler Features

The air sampler is a highly effective multi-stage wetted-wall cyclone that continuously processes air at a 325 LPM rate, extracting particulates and transferring them to a liquid phase of 4 to 5 cc volume (see Figure 2). Distilled water is typically the liquid of choice; no additives or surfactants are required for maximum efficiency. This liquid portion may then be periodically transferred in part or in whole to the biodetector, using a built-in peristaltic pump. A dropper bottle filling station is also integrated into the BioHawk so that a portion or all of the sample may be dispensed into the dropper bottle for archiving and/or application of an alternative analysis method. For example, the dropper bottle feature allows the BioHawk to be used as a sample collection and preparation system for lateral flow tickets. Unique and patented features of the air sampler include its abilities to operate unattended for long periods of time and to amplify trace analyte concentrations by maintaining a user-selected sample fluid volume in the device, independent of collection time, air temperature or relative humidity.

This is accomplished by monitoring liquid inventory with a proprietary sensor attached to the cyclone body. When the sample water inventory falls below the set point, the sensor initiates clean water transfer into the cyclone body from an onboard 1 liter water supply. Water inventories may be maintained within a recommended range of about 4 cc to 5 cc with an accuracy of a few tenths of a cubic centimeter.

Air sampler flow schematic

Figure 2: Air sampler flow schematic.

Biodetector Description

The instrument's biodetector section consists of a disposable 8-channel fluorometric assay coupon (see Figure 3) suitable for the high-sensitivity detection of biological agents, toxins, explosives, and chemical contaminants. All target-specific reagents needed to perform an assay are contained within the coupon. The only fluid not carried in the coupon is a saline buffer used to wash the system between assays. It is stored within a refillable reservoir in the instrument and a waste water reservoir is also provided. This ensures that no fluids are discharged from the instrument during either air sampling or the bioassay step.

Photo of BioHawk bioassay coupon

Figure 3: BioHawk bioassay coupon.

Upon coupon insertion into the instrument, an optical bar code on the coupon is automatically interrogated for assay recipe information. Highly reliable computer-controlled peristaltic and syringe pumps are used to move reagents, sample fluids and wash buffer within the coupon, as directed by the assay recipe. Automation of the assay process in this way ensures repeatable behavior from test to test.

Targeted agents are detected by monitoring fluorescently-tagged chemical reporter reactions taking place on optical waveguide surfaces within the coupon (see Figure 4). These reactions typically use antibodies to bind targeted pathogens to the waveguide surfaces, and fluorophore-tagged secondary antibodies to create a fluorescent signal when the waveguides are irradiated with 635 nm solid state laser light. See the RAPTOR Sandwich Assays chart for additional information on waveguide-based biosensing and analytes that can be detected with this type of approach.

Assay data is automatically stored in the system's non-volatile EEPROM with a date and time stamp, and can be downloaded to a remote computer using an RS-232 or wireless link. The BioHawk has the capacity to save up to 6,000 data sets.

Auxiliary Windows-based software allows the user to graphically monitor data recovery while an assay is running. It also provides sophisticated users with the ability to customize the various steps involved in running an assay.

Illustration of evanescent wave biodetection process

Figure 4: Evanescent wave biodetection process.

 

Front illustration of BioHawk components

Figure 5: BioHawk components (front view illustration).

Rear view illustration of BioHawk components

Figure 5: BioHawk components (back view illustration).

This product is covered by one or more of the following patents: U.S. Patents No. 6,136,611; 5,430,813; 6,082,185; 6,532,835; 5,061,857; and Japanese Patents No. 3,429,282 and 3,754,440.

Software Interface

The BioHawk Program runs in a single main window entitled ‘BioHawk’ which contains a menu and Toolbar at the top, and a status panel at the bottom. The Main window (see Figure 7-1) can be minimized or reduced in size without affecting operation or data acquisition. Important messages requiring manual input will continue to be displayed even when the program is minimized.

BioHawk Program Main Window
Figure 7-1: The Main window.

BioHawk General Specifications
Characteristic Description
Use Profile: Indoor/outdoor sample collection, transfer, and assay; storage of 255 assay recipes; user in full MOPP gear either walking or in moving vehicle.
Collection principle: Multi-stage wetted-wall cyclone with enhanced particulate collection.

Assay method: Disposable wet assay coupon-reuseable up to 10 times. Eight simultaneous software-based assays. Antibody or nucleic acid. Coupon reseals on removal for archival storage.
Fluid Handling: Fluids manipulated under microprocessor control using peristaltic and syringe pumps; sample may be oscillated to lower assay time; reagent is recovered for reuse.
Fluids storage: Snap on 3-section fluid pack. Clean water: 1 liter; Buffer: 250 ml; Waste: 500ml
Human interface: Day/night Touchscreen LCD display, usable in MOPP gear.
Digital communication: RS-232 bi-directional serial link
Physical size: 35.6 cm W x 36.5 cm H x 17.1 cm D
Weight: 20.9 lbs. dry; 25.9 lbs. with battery and fluids (9.5/11.8 kg).
Operating/storage: 1 to 66°C and -29 to 66°C. Reagent deterioration can reduce upper limit significantly.
Humidity: 10% and above. May be operated in rain.
Survivability: MILSPEC 810F; MTBF of about 30,000 hours is determined by air sampler fan.
Data storage: Flash memory retains raw/processed data for over 6000 assays.
Power Consumption: 5.6 W at idle; 17.5W with fan operating and one assay performed each 30 minutes.
Power source: Primary battery BA-5390A/U, 1.05 kg (2.3 lb); lifetime 14 to 45 hours.
Rechargeable battery UBI-2590; lifetime is approximately 56% of the BA5390A/U primary battery.
Universal lump-in-cord power supply, 82-265 Volt (47-63 Hz).
Alarm: Visual LED and 103 dB @0.6m waterproof horn; adjustable. RS-232 data link.
Decontamination:

Auto-flush protocols using onboard water, or manual flush with detergent and/or disinfectant. High-performance pull-through fan easily removed if contaminated.
Sound level: 60 dB (A).
Ancillary equipment: Heavy-duty hard-shell transport case with wheels.

BioHawk Frequently Asked Questions

Is it possible to quantify the BioHawk assay analysis?

Yes. What you do is challenge the coupon with a range of concentrations of the target analyte, and in that way obtain a response curve. While the unit will not compute concentrations directly, the data can be downloaded and the data compared with the response curve. If you had a customer that was going to buy a large number of units, we could provide custom software (hopefully, paid for by the user) that allowed you to load a response curve into the BioHawk (or RAPTOR) and which would provide a numeric output. Users generally prefer to stay away from numeric outputs, as field operators usually are more interested in and capable of understanding presence/absence, rather than actual concentration.

 

Please explain in detail why the disposable waveguide probes can be re-used up to 15 times? Once a virus is detected does the waveguide probe need to be thrown away?

The waveguides are coated with a target-specific antibody. If the target is not present in a sample, the coating is still good, and is available for further sample challenges. The coating is stable for up to 24-48 hours, depending on temperature, the presence of chemicals that might cause the antibodies to become damaged, or bacteria that might like to eat the antibody coating. The secondary fluorescent reagent is stored and reused, so its life is long as well. It does not attach to the waveguide unless the target substance is present in the sample, so it is not depleted except through dilution. cause by mixing with water fillets in the coupon's waveguide portion.

Once one of the waveguides has captured some of its targeted substance, that particular waveguide is compromised, but the other waveguides (assuming they target something else) would still be unaffected. Subsequent assays may show a small positive response on that channel, even if there is no targeted substance present in later samples, due to the fact that the secondary antibody reaction is not 100% effective at labeling each captured target on the first use. This is a conscious choice on our part. By not going to equilibrium on the step where the waveguide is soaked in the secondary antibody, the assay time is significantly shortened.

 

Please explain the definition of "detection", "monitoring", "confirmatory analysis", "classification" and "identification" and what are the differences between these four?

Detection means that something is being found present. It is a broad term, and doesn't say anything by itself, as to the concentration, etc. A simple example might be your nose detecting an odor. You may or may not know what the odor corresponds to, but there is enough odor for your nose to 'detect' it. A biotrigger might for example detect the presence of some type of spore in the air, while a bioidentifier will not only detect the spore, but tell you what type of spore it is.

Monitoring means that something is being continuously done, as in monitoring the speed of your car. Most biotriggers monitor for a class of material, such as organic vs inorganic, or bacteria vs spores, by continuously drawing air through a detection volume. Most of these devices use an ultraviolet light beam that responds in a specific way to the classes they can distinguish. Our bioidentifier's air sampler also continuously monitors, that is, it draws air in and places the particulates in a small amount of water. It differs from a typical trigger in that specific pathogens or toxins can be identified in about 15 minutes after a sample has been taken from the collector's water inventory. So our bioidentifier in essence keeps a record of what particles it has seen because they continue to circulate in the sampler's water until you pull some of it off either for analysis; for transfer to the onboard bottle for a confirmatory analysis; or you wash the sampler out.*

Confirmatory Analysis means an analysis that uses another method for detecting a pathogen or toxin. All portable instruments are considered to be warning devices, and the presence of a partricular target is usually confirmed later by using some widely accepted microbiological method, such as culturing in a petri dish. If a portable detector does not have a way of saving a sample for later analysis, it is usually considered deficient. Any secondary analysis method that does not use the same detection principles as the portable bio-identifier will be considered a 'confirmatory analysis.' For example, bioassay tickets do not use the same assay method as the BioHawk, so a 'second opinion' with a ticket analysis would be, for some cases, an adequate confirmatory method. However, purists always want to grow the bugs in a petri dish at some later point. In the case of the SBS, it's air sampling rate is so low that you have to question its ability to gather a large enough sample from any cloud it passes through, to provide a good confirmatory analysis.

Classification means, in the present context, that the device helps to sort out what kind of material is present in the air, at whatever detection limits it has, but it will not identify specific pathogens or toxins. As an example, air quality indexes on television typically give you pollen count. In this case, the device that gives you pollen count does not tell you what type of pollen it is- just the total. That is the same situation with biotriggers, which are classifiers. They only give you a reading related to the total quantity of each target type.they can differentiate between- not pathogen versus non-pathogen, for example. Most triggers that cost less than $100K primarily give a response that tells you the amount of organic material suspended in the air as opposed to inorganic material (such as sand). The idea is to give a warning that some type of bio-identification should be done. A big problem with them is that if the total concentration of a particular class stays roughly the same, no alert is given.

 

We use sandwich assays to detect viruses, do we use the same principle to detect chemicals like Sarin?

Sarin is too small a molecule to be detected. Usually, a molecule has to have a molecular weight of over 500 to have enough surface structure to be recognized by an antibody. Sarin only has a molecular weight of 140. It might be possible to engineer non-antibody based assay reactions for analytes such as Sarin, but such an option is not currently offered.

 

Please explain the difference between array, coupon and recipe.

An "array" is usually a grouping of discrete chemical reaction sites.

A "coupon" is a disposable credit card-sized assay module that has fluidics and chemistry integrated together. It would have an array of capture antibody sites and fluidic channels connecting the sites to the instrument. In the BioHawk coupon, it additionally stores the secondary antibody reagent in small onboard reservoirs.

A "recipe" is a series of instrument operations that together, allow a user to perform an assay under total computer control. In general, these operations include turning pumps and valves on and off for programmed times, turning interrogating lasers on and off for programmed times, storing samples for confirmatory analysis, and performing aerosol sample collection for a programmed time.

 

What are the detection parameters for BioHawk?

The BioHawk measures the total fluorescent light produced by dye molecules captured onto the waveguide surface. These dye molecules are captured during the bioassay process, if the target molecule or organism was first captured by the sensing surface. There are arrays of photodiodes under the bioassay coupon. This fluorescent light, after passing through an optical filter that removes most of the exciting laser light, impinges on the photodiode arrays and is converted to photocurrents by the photodiodes. So the ultimate measured electronic parameter is an electrical current, while the physical parameter being monitored is the number of dye molecules attached to the capture surface.

Calibration is done by challenging the device with different concentrations of the target, and then getting a plot of signal versus concentration. Most users aren’t interested in quantitative results, so we parse the response into “negative,” “suspect,” “positive,” and “high positive.”

What is the life cycle of one BioHawk coupon when detection result is “positive,” “negative” or a combination of both? How do you calculate coupon life cycle? And how do you define the start and an end of a reaction?

A ‘"Channel" area set aside for each specific target. The optics are set up to focus eight different sensing areas onto eight different sets of photodiodes. Hence, the BioHawk can be set up to simultaneously monitor up to 8 "channels" or equivalently, eight different targets. If you do 10 measurements on a coupon, then the number of measurements per target is 10, but the total number of measurements for all channels is 8 x 10 = 80 separate bioassays.

The surface reactions we harness to detect targets are not run to completion because that would take too much time. Instead, we allow (through the microprocessor-controlled fluidics) the liquid sample and the reagents to contact the sensing surfaces for a precise amount of time that is less than the amount needed for the reporter reactions to go to completion. This allows an assay to be completed in 15 minutes- as compared to 0.5 hr to 1 hour if we were allowing everything to equilibrate at each step.

The number of assays that can be performed is limited by dilution of the reagent liquid onboard the coupon. We have four small reagent reservoirs of about 0.35 ml each on the coupon. Every time reagent is used inside the coupon, it is pumped out onto the sensing surfaces. After a few minutes, it is pumped back into the reservoirs. Every time you do this, small fillets of water in the sensing area mix with the reagent and dilute it a little. After 10 to 15 assays, the reagent concentration has begun to drop a little and the assay may not be as sensitive. The dilution rate is somewhat variable, so that is why you might find both 10 and 15 assays noted in different places. To be safe, you should not quote more than 10 runs per coupon.


Our target reagents include biological agents, toxins, explosives and chemical contaminants. Can one coupon hold a mixture of 4 different classes of target reagents at once?

Yes, there is no problem with different classes. But note that the classes we have assays for right now are toxins, viruses, bacteria, spores, and microorganisms; not explosives or chemicals. There are other methods for those targets that are probably better, unless there are unusual circumstances.


Is BioHawk able to detect and identify the newest flu, the H1N1?

While the air sampler portion can collect the H1N1 virus, we do not have an assay for it.


How large an area can BioHawk cover for sampling? For example, if a room has dimensions of 10 feet by 10 feet by 10 feet that needed to be cleared and how many BioHawks would need to be present to finish the clearance in one cycle (i.e 15 min.)?

If you want to sample all the air in a room once, and do it in a finite time, then you need a high volume concentration device like the SASS 4000 or SASS 4100, which collect at a rate of about 3,600 LPM. At the SASS 2300's 325 LPM, it would take over 80 minutes to do one air turn, while the SASS 4000 would do one turn in about 8 minutes. However, micron-sized pathogens will tend to diffuse and float about, even in a closed space, and it is not usually necessary to sample all the air to find an aerosol pathogen. The minimum-size air sample is instead dictated by the sensitivity of the assay used, the air concentration of the pathogen, the liquid sample size, the sampling time, and the volumetric sampling rate. Some assay methods are sensitive to the total number of pathogens captured, but most have a detection limit that can be characterized as a minimum pathogen concentration in the liquid sample. So you want to do whatever you can to increase the pathogen's concentration in the liquid. You can do this by increasing the air flow rate, so long as it does not adversely affect capture efficiency; decrease the liquid sample volume; and/or increase the sampling time.


Can we expect an extended lifetime of a coupon over 24 hours after hydration when no bio-assay is done during the period?

You may expect some prolonged usage time. However, Research International cannot assure performance after 24 hours. Usage time is prolonged if system operation is below 20° C, and is shortened at higher temperature. We have tested coupons up to 50° C, and the 24-hour lifetime is conservative. Let us know if a more detailed relationship between operating temperature and lifetime is needed.

 

If there are flurophore floating in the room while the assay process is taking place, will it affect the result? If not, how does the coupon keep flurophore away?

There just aren't significant amounts of fluorescent material floating around in the air that can be stimulated by 632 nm excitation light, so unless you're in a fluorescent paint factory, I don't think this issue will ever arise in real world aerosol sampling!   The fluorophores  used in the coupon are  specially designed to  efficiently  fluoresce  with 632 nm excitation, and there just aren't very many natural aerosols that  will  confound  the instrument.

There may be some fluorescent algae that could be detected by the instrument through their solution fluorescence, but  the instrument doesn't actually take any readings while the sample is incubating on the waveguide.  The  before-and-after fluorescent readings that determine whether something has been captured are only taken while the coupon is filled with clean buffer from the storage reservoir.  Buffer fluid is used to flush out any fluorescent species that were present during the sample incubation step.  In other words, there are no readings taken during the time the sample is resident in the coupon, so any residual fluorescent material should be at a very low level.

BioHawk Collector/Bioidentifier Parts and Accessories

This page is a form. Check the Select Box by any item for which you would like a quote. Complete the form information at the bottom of the page and press Submit. We try to respond within 24 to 48 hours.


BioHawk System. Part Number: 7000-156-400-02 BioHawk® System

Part Number: 7000-156-400-02

Includes:
  • BioHawk
  • Cleaning coupon
  • Sample syringes (5)
  • RS-232 cable for BioHawk to PC communications
  • Voltage adapter (wall plug transformer, AC/DC)
  • BA-5590B/U primary battery *
  • Instruction Manual
  • Field Operator's Checklist
  • Software on CD-ROM (Requires Windows Server 2003/2008, Vista or XP operating system).

* Except Europe where due to IATA International Regulations we are no longer able to offer optional battery supplies. Customer will be advised as to battery models and suppliers upon placement of order.

 

Replacement primary battery. Part Number: 1000-0003Replacement Primary Battery (BA-5590B/U)*

Part Number: 1000-0003

 

* IATA International Regulations prevent us from offering optional battery supplies to international customers. Customer will be advised as to local battery models and suppliers upon placement of order.

 

 

 

Replacement Long-Life Battery. Part Number: 1000-0003-15Replacement Long-Life Battery
(BA-5390A/U)
*

Part Number: 1000-0003-15

 

* IATA International Regulations prevent us from offering optional battery supplies to international customers. Customer will be advised as to local battery models and suppliers upon placement of order.

 

 

 

 

Rechargeable Lithium Ion battery. Part Number: 1000-0003-12Rechargeable 15V Lithium Ion Battery*

Part Number: 1000-0003-12

 

* IATA International Regulations prevent us from offering optional battery supplies to international customers. Customer will be advised as to local battery models and suppliers upon placement of order.

 

 

 

 

Charger for Lithium Ion battery. Part Number: 1000-0003-13

Charger for lithium ion battery

Part Number: 1000-0003-13

 

 

Solar powered battery charger. Part Number: 1000-0004-01

Solar powered battery charger

Part Number: 1000-0004-01

 

 

Fully assembled coupons with antibodies. Part Number: 7100-156-700-xxFully assembled coupons with antibodies

Part Number: 7100-156-700-xx

Minimum order: 10

Please e-mail or call regarding your needs for coupons. Any mix of ricin, anthrax, ovalbumin, and/or BG specific waveguides BioHawk disposable coupon format. Others quoted on request. Each coupon may be reused 10-15 times (depending on conditions) if no target pathogen is detected. May order from 1 to 8 channels.

Note: All coupons are presently research-grade products and performance may vary from lot-to-lot.

 

BioHawk cleaning coupon. Part Number: 7100-156-800-01Cleaning Coupon

Part Number: 7100-156-800-01

 

 

 

BioHawk training coupon. Part Number: 7100-156-801-01Training Coupon

Part Number: 7100-156-801-01

 

 

 

BioHawk buffer solution kit. Part Number: 7000-156-570-01Buffer Solution Kit

Part Number: 7000-156-570-01

Includes: Buffer Solution Part A and Part B

 

 

 

 

 

 

Sample syringe. Part Number: 7100-070-181-01Sample syringe

Part Number: 7100-070-181-01

For loading samples into the BioHawk; 3 cc syringe with blunt needle.

 

BioHawk transport case. Part Number: 7100-079-280-02

BioHawk Transport Case

Part Number: 7100-079-280-02

Rugged Pelican Transport Case (padded, ruggedized carry case for transport and protection of BioHawk. Includes collapsible handle and wheels for easy transport.

 

 

 

 

 

BioHawk replacement brushless fan. Part Number: 7200-156-714-01Replacement brushless fan

Part Number: 7200-156-714-01

 

 

 

 

 

BioHawk 11V power supply. Part Number: 7200-156-700-01110V Auxiliary Power Supply

Part Number: 7200-156-700-01

AC/DC assembly transformer, up to 300V

 

 

 

 


RS-232 cable. Part Number: 0900-0003RS-232 Cable

For BioHawk to PC communications

Part Number: 0900-0003

 

 

 


BioHawk backpack assembly. Part Number: 7000-156-406-01BioHawk backpack assembly

Part Number: 7000-156-406-01

 

 

 

BioHawk Fluids Module three tank replacement assembly. Part Number: 7100-156-402-04Fluids Module three tank replacement assembly

(Customer to supply product serial number)

Part Number: 7100-156-402-04

 

 

BioHawk replacement filter screen. Part Number: 2000-156-321-01

BioHawk Replacement Filter Screen

Part Number: 2000-156-321-01

 

 

USB to RS-232 adapter. Part Number: 7000-0004-01

USB to RS232 Adapter

Part Number: 7000-0004-01

 

 

 

BioLink Bluetooth wireless. Part Number: 7000-0003-02BioLink Bluetooth Serial
Data Radios

Part Number: 7000-0003-02

Includes:
  • Set of two (2) serial BioLinks (DB-9 style) with null modem adapters and USB power cables
  • Quick Start Guide
  • Two (2) +5dBi right angle, right hand antenna (400 meter range. Shown above).
  • Two (2) +1dBi Stub Antenna (100 meter range)
  • CD with software and documentation
 

BioLink Bluetooth wireless. Part Number: 7000-0003-03BioLink Bluetooth Serial/USB
Data Radios

Part Number: 7000-0003-03

Includes:
  • One (1) Bluetooth serial adapter with null modem adapter and USB power cable and one (1) USB Bluetooth adapter
  • Quick Start Guide
  • Two (2) +5dBi right angle, right hand antenna (400 meter range. Shown above).
  • Two (2) +1dBi Stub Antenna (100 meter range)
  • CD with software and documentation
 

BioLink Bluetooth wireless. Part Number: 1100-0016-08+5dBi Dipole right angle, right hand antenna (400 meters)

Part Number: 1100-0016-08

 

 

BioLink Bluetooth wireless. Part Number: 1100-0016-09+9dBi Patch antenna (1,000 meters)

Part Number: 1100-0016-09

 

 

 

 

 

 

BioLink Bluetooth wireless. Part Number: 1100-0016-07Single BioLink Bluetooth Serial Data Radio

Part Number: 1100-0016-07

Includes:
  • One (1) Bluetooth serial adapter
  • Null modem adapter
  • USB power cable and one
  • One (1) +1dBi Stub Antenna (100 meter range)
  • Quick Start Guide
  • CD with software and documentation

 

 

BioLink Bluetooth wireless. Part Number: 1100-0016-10Single BioLink Bluetooth USB Data Radio

Part Number: 1100-0016-10

Includes:
  • One (1) USB Bluetooth wireless device
  • One (1) +1dBi Stub Antenna (100 meter range)
  • Quick Start Guide
  • CD with software and documentation
 

BioLink Bluetooth wireless. Part Number: NoneBioLink Base Stations

Available in 7, 14, and 28 Bluetooth connection models (purchase BioLink units separately)

Base Station A - 7 Nodes

Base Station B - 14 Nodes

Base Station C - 28 Nodes


 

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